Purification of peptides and oligonucleotides: puriFlash® 5.250P

Presentation | Technical specifications | Configurator

Purification of peptides and oligonucleotides: puriFlash® 5.250P

Presentation | Technical specifications | Configurator

Purification of peptides and oligonucleotides puriFlash 5.250P

Non-contractual picture.
System delivered without pump door.

puriFlash® 5.250P

Optimized for your Flash and preparative purification of complex mixtures of peptides and oligonucleotides.

Routine Flash purification puriFlash XS 520 Plus interchim

Flow rate
125 mL/min

Routine Flash purification puriFlash XS 520 Plus interchim

Max. pressure
250 bar

  • Small organic molecules 85% 85%
  • Impurity detection 100% 100%
  • Natural products 100% 100%
  • Peptides and oligonucleotides 100% 100%
  • Proteins and biopharmaceuticals 65% 65%
  • Isolation of metabolites 98% 98%
  • Trace enrichment 100% 100%

Components

  • Control unit
    Pump
    UV detector
    Fraction collector
    Solvent tray with drainage system
    2 integrated column holders or 3 column holders (included in Pack-Multi)
    2 integrated Dry-Load holders

Pump

  • Flow rate range : 1 mL/min to 125 mL/min
    Max. operational flow rate : 125 mL/min
    Optimum flow rate for columns up to 50mm id
    Flow rate accuracy:: +/- 2 % over 5 mL/min to 100 mL/min & 5 to 200 bar
    Flow rate repeatability : < 0.25
    Flow rate linearity : 0.98 < linearity < 1
    Pressure range : 0 to 250 bar
    Max. operational pressure: 250 bar
    Quaternary gradient valve
    Gradient tolerance step: < 3.5 %
    Air purge
    Washing discs
    Washing pump (allows circulation of water to prevent crystalline deposits on the pump piston)

Injection

  • Injection mode: liquid or solid
    “Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or loops for even more efficient injection
    “RFID technology”: automatic detection of dry load and injection loops
    Stainless steel injection loops 2 mL and 5 mL with RFID tag

Detection

  • UV detector 200-400 nm
    UV detector 200-800 nm (included in the Pack-UVextended)
    “DAD” technology
    Multi-wavelength and scan collection over a band of 200 nm (or 600 nm with the Pack-UVextended)
    Spectral visualization and confirmation of purity
    Flow cell pathway: 1.3 mm / 55 µL
    Integrated ELSD detector (included in the Pack-iELSD)

Column & holder compatibility

  • Normal phase (NP)
    Particle sizes: 5 µm, 10 µm, 15 µm, 25 µm, 30 µm, 50 µm
    Reverse phase (RP)
    Particle sizes: 5 µm, 10 µm, 15 µm, 25 µm, 30 µm, 50 µm

    Format: all Flash columns up to F0080 & Dry-Load up to F0080 are compatible
    all preparative column up to 50mm id are compatible

    2 integrated column holders or 3 column holders (included in Pack-Multi)
    2 integrated Dry-Load holders

    RFID technology: automatic detection of the columns

Fraction collector

  • Capacity:
    – 3 racks
    – 132 tubes (18 x 150 mm)

 

  • Technology:
    – Automatic cleaning of the fraction collector needle (to avoid cross-contamination with the previous purification)
    – RFID: automatic detection of the racks installed in the fraction collector

Proposed racks

  • Delivery includes a choice of 3 racks from the following list:
  • ● Rack for test tubes:
    13 x 73 mm | 13 x 100 mm | 16 x 150 mm
    18 x 150 mm | 18 x 180 mm | 21 x 150 mm
    25 x 150 mm | 28 x 150 mm | 29.5 x 200 mm
  • ●   Rack for 250 mL Schott bottles

    • Rack for funnels (in option)

    Position of the numbered tubes (to facilitate identification of tubes)

Software & control unit

  • Control via InterSoft ® X software included:
    • Genius, Artificial Intelligence
    • “Flash & Go”™ technology: compatible with our smartphone app “TLC to Flash & Prep”
    • “Boost & Go”™ technology
    Windows 10
    Remote control
    Touchscreen 15”
    8 USB ports
    2 RJ45 ports
    Bluetooth/Wi-Fi key

System optimization

  • Tubing after pump: PEEK 0.75 mm internal diameter
    Flow cell pathway: 1.3 mm / 55 µl

Safety

  • System light status
    Solvent tray with drainage system
    Level sensor for solvents and waste

    Retention tray with leak detectors:

  • – for the pump,

  • – for UV detector

  • – for the valve, Dry-Load holder

  • – for the fraction collector

  • – for the fraction  iELSD (included in the Pack-iELSD)

    “Fume Enclosure” technology (allows the use of the purification system without a hood)

Materials in contact with solvents

  • ETFE
    PTFE
    316 L Stainless steel
    Hastelloy
    PEEK
    Kalrez
    Sapphire
    Ruby
    Kel-F
    Zirconium
    PPS
    Nitronic 60
    Borosilicate glass

Accessories box

  • Tubing with fitting & ferrules:
    – Tygon tubing for solvent tray drainage
    – Level sensor tubing and waste (x5)
    – Suction tubes (x4)
    – Stainless steel adapter for injection (injection valve port 1)
    – ETFE tubing 1/8″ fraction collector outlet to waste
    – ETFE tubing 1/8″ draining for fraction collector
    – 2 ml & 5 ml Stainless Steel loop with RFID tag

    5.250P Flash version: tubing 1/16’’ od – 0.75mm id
    – PEEK tubing column C2 outlet to 6-ways valve port 3
    – ETFE tubing 6-ways valve port 4 to detector inlet
    – PEEK tubing column C1 outlet to 6-ways valve port 5
    – PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
    – PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
    – PEEK tubing 10-ways valve port 3 to inlet column C2
    – PEEK tubing 10-ways valve port 5 to inlet Dryload DYL2
    – PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
    – ETFE tubing 10-ways valve port 6 to waste
    – PEEK tubing 10-ways valve port 9 to inlet column C1

    5.250P Pack-Multi Flash version: tubes 1/16’’ od – 0.75mm id
    – PEEK tubing column C1 outlet to 6-ways valve port 5
    – PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
    – PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
    – PEEK tubing tubing 10-ways valve port 5 to inlet Dryload DYL2
    – PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
    – PEEK tubing 6-ways selection valve port 2 to column C3 inlet
    – PEEK tubing 6-ways selection valve port 6 to column C2 inlet
    – ETFE tubing 10-ways valve port 6 to waste
    – PEEK tubing 10-ways valve port 9 to column C1 inlet
    – PEEK tubing column C3 outlet to 6-ways selection valve port 3
    – PEEK tubing column C2 outlet to 6-ways selection valve port 5

    5.250P Prep version: tubing 1/8’’ od – 0.75mm id
    – PEEK tubing column C2 outlet to 6-ways valve port 3
    – PEEK tubing column C1 outlet to 6-ways valve port 5
    – PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
    – PEEK tubing 10-ways valve port 3 to inlet column C2
    – PEEK tubing 10-ways valve port 5 to inlet Dryload DYL2
    – PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
    – PEEK tubing 10-ways valve port 9 to column C1 inlet
    – PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
    – Adapter 5/16″-24 female to 10-32 male   
    – Stainless steel union 1/8″

    5.250P Pack-Multi version Prep: tubing 1/8’’ od – 0.75mm id
    – PEEK tubing column C1 outlet to 6-ways valve port 5
    – PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
    – PEEK tubing tubing 10-ways valve port 5 to inlet Dryload DYL2
    – PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
    – PEEK tubing 6-ways selection valve port 2 to column C3 inlet
    – PEEK tubing 6-ways selection valve port 6 to column C2 inlet
    – PEEK tubing 10-ways valve port 9 to column C1 inlet
    – PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
    – PEEK tubing column C3 outlet to 6-ways selection valve port 3
    – PEEK tubing column C2 outlet to 6-ways selection valve port 5
    – Adapter 5/16″-24 female to 10-32 male
    – Stainless steel union 1/8″

    ELSD accessories (included in Pack-iELSD)
    – Reduction gear tubing 6 mm to tubing 4 mm
    – Pneumatic white NYLON tubing length 3 m
    – In-line filter 20 microns
    – ETFE tubing from in-line filter to detector
    – ETFE tubing 6-ways selection valve port 4 to in-line filter

    PWD accessories:
    – Tubing kit PWD

    Cables :
    – RFID cable (x4)
    – USB/USB cable – unit control to fraction collector
    – Power cord – screen to fraction collector
    – HDMI cable – screen to unit control
    – USB/MINI USB cable – screen to unit control
    – LED cable – unit control to fraction collector
    – Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
    – Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
    – Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
    – Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in the Pack-iELSD)
    – Power cord – pump to fraction collector + unit control to fraction collector (x2)
    – General power cord

    Solvent tray
    Clips P
    Screw CHC M4x8
    Grower M4 (x3)
    Stylus with its clip
    UV cell test
    Rear fastening (x2)
    Screws BHC 4×10 (x2)
    UV trapdoor
    ELSD trapdoor
    Racks (x3)
    Compliance report

Communication

  • Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
    Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
    Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
    Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in the Pack-iELSD)

Power supply

  • 110 V or 220 V

Dimensions & weight

Purification of peptides and oligonucleotides puriFlash 5.250P interchim
  • Weight : 50 kg
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