Ultra-preparative: puriFlash® 5.400-UV

Ultra-preparative: puriFlash® 5.400-UV

Ultra-preparative puriFlash 5.400 chromatogra

Non-contractual picture.
System delivered without pump door.

puriFlash® 5.400-UV

Optimized for your ultra-preparative purifications

Routine Flash purification puriFlash XS 520 Plus interchim

Flow rate
125mL/min

Routine Flash purification puriFlash XS 520 Plus interchim
Max. pressure
400 bar
  • Small organic molecules 100% 100%
  • Impurity detection 100% 100%
  • Natural products 100% 100%
  • Peptides and oligonucleotides 85% 85%
  • Proteins and biopharmaceuticals 28% 28%
  • Isolation of metabolites 100% 100%
  • Trace enrichment 100% 100%

Components

  • Control unit
  • Pump
  • UV detector
  • Fraction collector
  • Solvent tray with drainage system

  • 2 integrated column holders

Pump

  • Flow rate range: 1mL/min to 125mL/min

  • Max. operational flow rate: 125mL/min

  • Optimum flow rate for columns up to 60mm ID

  • Flow rate accuracy: +/- 2.5 % from 3.5mL/min to 100mL/min & 5 to 320 bar

  • Flow rate repeatability: < 0.25

  • Flow rate linearity: 0.98 < linearity < 1

  • Pressure range: 0 to 400 bar

  • Max. operational pressure: 400 bar

  • Quaternary gradient valve

  • Gradient tolerance step: < 3.5 %

  • Washing discs

  • Washing pump allows circulation of water to prevent crystalline deposits on the pump piston (included in Pack-PWD)

Injection

  • Injection mode: liquid
  • “Load & Go” technology: with automatic 10-way valve that allows the use of loops for even more efficient injection

  • Stainless steel injection loops 2mL and 20µL

Detection

  • UV detector 200-400nm

  • UV detector 200-800nm (included in the Pack-UVextended)

  • “DAD” technology

  • Multi-wavelength and scan collection over a band of 200nm (or 600nm with the Pack-UVextended)

  • Spectral visualization and confirmation of purity

  • Flow cell pathway: 1.0mm / 15µL

Column & holder compatibility

  • Normal phase (NP)
    Particle sizes: 3µm, 5µm, 10µm, 15µm, 25µm, 30µm, 50µm

    Reverse phase (RP)

  • Particle sizes: 3µm, 5µm, 10µm, 15µm, 25µm, 30µm, 50µm

    Format: all preparative columns up to 60mm ID are compatible
    2 integrated column holders (holder n°1: col. ≥ 20mm ID – holder n°2: col. 7.8 – 10mm ID)

Fraction collector

  • Capacity:
    • • 3 racks
    • • 132 test tubes (18 x 150mm)

    Technology:

    • • Automatic cleaning of the fraction collector needle (to avoid cross-contamination with the previous purification)
    • • RFID: automatic detection of the racks installed in the fraction collector

Proposed racks

  • Delivery includes a choice of 3 racks from the following list:
  • • Rack for test tubes:
    – 13 x 73mm | 13 x 100mm | 16 x 150mm
    – 18 x 150mm | 18 x 180mm | 21 x 150mm
    – 25 x 150mm | 28 x 150mm | 29.5 x 200mm
  • • Rack for 250mL Schott bottles
  • • Rack for funnels (in option)
  • .

Position of the numbered tubes (to facilitate identification of the tubes)

Software and control unit

  • Control via InterSoft® X software included:
    • • Genius, Artificial Intelligence
    • • “Boost & Go”™ technology

     

  • Windows 10
    Remote control
    Touchscreen 15″
    8 USB ports
    2 RJ45 ports
    Bluetooth/Wi-Fi key

System optimization

  • Tubing after pump: 0.5 & 0.75mm internal diameter
    Flow cell pathway: 1.0mm / 15µL

Safety

  • System light status
    Solvent tray with drainage system
  • Level sensor for solvents and waste
    Retention tray with leak detectors
  •     • pump
  •     • column holder
  •     • UV detector
  •     • valve
  •     • fraction collector

“Fume Enclosure” technology (allows the use of the purification system without a hood)

Materials in contact with solvents

  • ETFE
    PTFE
    316 L Stainless steel
    Hastelloy
    PEEK
    Kalrez
    Sapphire
    Ruby
    Kel-F
    Zirconium
    PPS
    Nitronic 60
    Borosilicate glass

Accessories box

  • Tubing with fitting & ferrules:
    • • Tygon tubing for solvent tray drainage
    • • Level sensor tubing (x5)
    • • Suction tubes (x4)
    • • ETFE tubing collector to Waste
    • • SS tubing injection valve port 3 to C2 inlet
    • • SS tubing injection valve port 6 to waste
    • • SS tubing injection valve port 9 to C1 inlet
    • • PEEK tubing repartition valve port 3 to C2 outlet
    • • PEEK tubing repartition valve port 4 to UV cell inlet
    • • PEEK tubing repartition valve port 5 to C1 outlet
    • • PEEK tubing BPR outlet to collector inlet
    • • PEEK tubing UV cell outlet to BPR inlet
    • • SS port for injection (injection valve port 1)
    • • Loop 2mL
    • • Loop 20µL
    • • External UV cell 1mm PEEK
    • • BPR 100PSI
    • • Column holder clip 12mm
    • • UV cell support
    • • Union 1/16”

     

    PWD accessories:

    • • PWD Tubing Kit

     

    Cables :

    • • RFID cable (x4)
    • • USB/USB cable – unit control to fraction collector
    • • Power cord – screen to fraction collector
    • • HDMI cable – screen to unit control
    • • USB/MINI USB cable – screen to unit control
    • • LED’S cable – unit control to fraction collector
    • • Communication cable Sub-D9 male/female (1m) – unit control to fraction collector
    • • Communication cable Sub-D9 male/female (0.5m) – unit control to pump
    • • Communication cable Sub-D9 female/female (1m) – unit control to UV detector
    • • Power cord – pump to fraction collector + unit control to fraction collector (x2)
    • • General power cable (model according to the country of destination)

     

    Solvent tray
    Clips P
    Screw CHC M4x8
    Grower M4 (x3)
    Stylus with its clip
    UV cell test
    Rear fastening (x2)
    Screws BHC 4×10 (x2)
    UV trapdoor

  • iELSD trapdoor
    Racks (x3)
    Compliance report

Communication

Communication cable Sub-D9 male/female (1m) – unit control to fraction collector
Communication cable Sub-D9 male/female (0.5m) – unit control to pump
Communication cable Sub-D9 female/female (1m) – unit control to UV detector

Power supply

110V or 220V

Dimensions

Ultra-preparative puriFlash 5.400 size

Weight: 50kg

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