Detectors: UV-Vis
Detectors: UV-Vis
UV-Vis Detectors
200 – 400 nm and 200 – 800 nm (UVextended-Pack)
Multi-wavelength and scan collection
UV-Vis Detectors
200 – 400 nm and 200 – 800 nm (UVextended-Pack)
Multi-wavelength and scan collection
The UV detectors, which are fully integrated in the collection module, are controlled by the InterSoft X software. Thanks to the very simple application, it is sufficient to enter the desired wavelength into the software and “Genius” will take care of the acquisition and recording of the chromatograms.
Choose the UV-Vis detectors suited to your needs.
The UV-Vis detector supplied with the puriFlash® device allows you to select 4 wavelengths simultaneously, in the same manner as a SCAN (the total number of wavelengths available with the two models can be included in this one).
Each laboratory has different needs, and since we are anxious to meet those needs and always provide you the most appropriate solution, we propose two UV detectors:
200 – 400 nm (standard – except for the PF-020B intended for proteins)
200 – 800 nm (with UVextended pack for puriFlash® compatible with this option)
More about how the UV-Vis detector works
When a chemical function is subjected to radiation, it passes over into an excited state due to the absorption, making it is possible to define a collection via an absorbed signal. In order to be detected, the compound must have at least one chromophore in its structure (e.g., aromatic grouping, two conjugated double bonds …), so the signal is absorbed and a peak appears on the chromatogram.
Consequently the detection module measures the absorption of a liquid passing through the UV cell at a certain wavelength. The deuterium lamp emits a light beam which passes through the UV cell filled with a mixture of solvent and compounds. Using a grid, the light beam is sent back to the diode bar and the photomultiplier generates the signal.
Designed completely for your purifications:
Forget about the risks of satiety once and for all.
The UV-Vis detectors of your puriFlash® have been designed specifically for preparative purification and flash chromatography.
Risk of saturation? Do not give it another thought – they detect any amount of product, from the smallest to the largest, without any saturation whatsoever.
You require columns in formats F0001 to F1600 for your purifications? No problem – our UV-Vis detectors are tailored to all sizes.
UV scan over the entire wavelength
& UV spectrum in real time.
The UV-Vis detectors contained in the puriFlash® Interchim® are equipped with the “DAD” technology (Diode Array Detector). This provides approximately one hundred diodes for scanning the entire wavelength for your detection, thus making it possible to obtain the UV spectrum of the eluted compounds in real time.
If you wish, you can return to a peak of the chromatogram at any time (even during the run or in the reprocess) in order to display the spectrum at the selected time and to obtain the elements of identification and the purity of the purified compounds.
Learn more
Variable UV absorption – maximum sensitivity at all times
Thanks to the performance of our cells with optical path length
The more relevant the optical path, the easier it is to see the compounds which absorb little in the UV.
There are three types of UV cells available:
Standard configuration for puriFlash® purification systems (except PF-5.400 and puriFlash® intended for peptides) – Stainless steel
Standard configuration of puriFlash® 5.400 purification system – External cell – PEEK
Standard configuration of puriFlash® purification systems intended for peptides: PF-5.250P – Stainless steel
In no time you have access to the lamp and the UV cell
Our maintenance concept? Ultra-easy.
Your lamp or detection cell needs to be replaced?
The magnetic cover can be removed in an instant.
Everything is easily accessible without any additional tools.
Fully controlled via InterSoft X software.
The various parameters (signal detection, selection of wavelengths and the entire scan, automatic zeroing) are fully controlled by the InterSoft X software to ensure comfort and ease of use on a daily basis.
Increased safety:
Retention tank and leak detector.
“And if in the event that …”,any potential risks you may encounter is something that we always keep in mind during the design process of our instruments, meaning they are extremely safe. A retention tank is therefore provided to catch the solvents that could escape during a potential leak.
And what about “prevention being better than cure”? Should a problem occur, the leak detector signals for you to pause the purification system, thus preventing the solvent from spreading. No failure, no maintenance.
Increased safety:
Retention tank and leak detector.
“And if in the event that …”,any potential risks you may encounter is something that we always keep in mind during the design process of our instruments, meaning they are extremely safe. A retention tank is therefore provided to catch the solvents that could escape during a potential leak.
And what about “prevention being better than cure”? Should a problem occur, the leak detector signals for you to pause the purification system, thus preventing the solvent from spreading. No failure, no maintenance.
UV: 200 – 800 nm (UVextended pack)
