Process Flash purification : puriFlash® 535-XL

Process Flash purification : puriFlash® 535-XL

535-XL-Technical-Specification_2023

puriFlash® 535-XL

Optimized for your routine Flash purification

Routine Flash purification puriFlash XS 520 Plus interchim

Flow
400 mL/min

Routine Flash purification puriFlash XS 520 Plus interchim

Max. pressure
35 bar

  • Small Organic Molecules 97% 97%
  • Impurities Identification 85% 85%
  • Natural Products 85% 85%
  • Peptides & Oligonucleotides 55% 55%
  • Proteins & Bio-drugs 20% 20%
  • Metabolites Isolation 20% 20%
  • Traces Enrichment 97% 97%

Components

  • Control unit
  • Pump
  • UV detector
  • Fraction collector
  • Solvent tray with drainage system
  • Integrated column holder (included in the Pack-ColHolder)
  • Stainless steel needle with connection (54 mm)

Pump

  • Flow rate range: 10 mL/min to 400 mL/min
  • Max. operational flow rate: 400 mL/min
  • Optimum flow rate for columns up to F1600 format
  • Flow rate accuracy: +/- 2 % over 20 to 320 mL/min & 5 to 28 bar
  • Flow rate repeatability: < 0.25
  • Flow rate linearity: 0.98 < linearity < 1
  • Pressure range: 0 to 35 bar
  • Max. operational pressure: 35 bar
  • Quaternary gradient valve
  • Gradient tolerance step: < 3.5 %
  • Air purge

Injection

  • Injection mode: liquid or solid

Detection

  • UV detector 200-400 nm
  • UV detector 200-800 nm (included in the Pack-UVextended)
  • ”DAD” technology
  • Multi-wavelength and scan collection over a band of 200 nm (or 600 nm with the Pack-UVextended)
  • Spectral visualization and confirmation of purity
  • Flow cell pathway: 0.3 mm / 40µL

Column & holder compatibility

  • Normal phase (NP)
    Particle sizes: 10 µm, 15 µm, 25 μm, 30 μm, 40 μm , 50 μm
  • Reverse phase (RP)
    Particle sizes: 15 µm, 30 μm, 50 μm.
  • Format: all Flash columns up to F0330 format are compatible
  • Integrated column holder (included in the Pack-ColHolder)

Fraction collector

  • Capacity:
     2 long racks
     112 test tubes (18 x 150 mm)
  • .
  • Technology:
    Automatic cleaning of the fraction collector needle (to avoid cross-contamination with the previous purification)

Proposed racks

  • Delivery includes a choice of 2 racks from the following list:
  •  Rack for test tubes:
    13 x 73 mm | 13 x 100 mm | 16 x 150 mm
    18 x 150 mm | 21 x 150 mm | 25 x 150 mm
    28 x 150 mm | 29,5 x 200 mm
  •  Rack for 250 mL Schott bottles
  •  Rack for funnels (not available with choice of device)

Software and control unit

  • Control via InterSoft® X software included:
  • Genius, Artificial Intelligence
  • ”Flash & Go”™ technology: compatible with our smartphone app ”TLC to Flash & Prep”
  • ”Boost & Go”™ technology
  • Windows 10
  • Remote control
  • Touchscreen 15″
  • 6 USB ports
  • 4 RS232 ports
  • 1 RJ45 port
  • Bluetooth/Wi-Fi

System optimization

  • Tubing after pump : 1.6 mm & 2,4mm internal diameter
  • Flow cell pathway: 0.3 mm / 40 µL

Safety

  • Solvent tray with drainage system
  • Retention tray in the pump
  • Retention tray with drainage system in the fraction collector
  • ”Fume Enclosure” technology (allows the use of the purification system without a hood).

Materials in contact with solvents

  • ETFE
  • PTFE
  • 316 L Stainless steel
  • Hastelloy
  • Peek
  • Kalrez
  • Sapphire
  • Ruby
  • Kel-F
  • Zirconium
  • PPS
  • Nitronic 60
  • Borosilicate glass

Accessories box

  • 2 long racks
  • PEEK needle
  • Adapter ETFE luer male ¼-28 male – ID=1.6 mm
  • 250 mL Schott bottle
  • Adapter for 250 mL Schott bottle
  • TYGON tubing with tee for drainage system
  • UV cell test
  • Stylus with its clip
  • General power cable (model according to the country of destination)
  • Unit control power cable (model according to the country of destination) – present on the system
  • Compliance report

Communication

  • Communication cable Sub-D9 male/female
  • Communication cable Sub-D9 female/female

Power supply

  • 110 V or 220 V

Dimensions & Weight

Routine Flash purification puriFlash XS 520 Plus size
  • Weight: 36 kg

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