Preparative HPLC: puriFlash® 5.250
Preparative HPLC: puriFlash® 5.250
Non-contractual picture.
System delivered without pump door.
puriFlash® 5.250
optimized for your preparative purifications
250 ml/min
250 bar
Components
- Control unit
Pump
UV detector
Fraction collector
Solvent tray with drainage system
2 integrated column holders or 3 column holders (included in Pack-Multi)
2 integrated Dry-Load holders
Pump
- Flow rate range : 1 mL/min to 250 mL/min
Max. operational flow rate : 250 mL/min
Optimum flow rate for columns up to 80mm id
Flow rate accuracy:: +/- 2 % over 20 mL/min to 250 mL/min & 5 to 200 bar
Flow rate repeatability : < 0.25
Flow rate linearity : 0.98 < linearity < 1
Pressure range : 0 to 250 bar
Max. operational pressure: 250 bar
Quaternary gradient valve
Gradient tolerance step: < 3.5 %
Air purge
Washing discs
Washing pump allows circulation of water to prevent crystalline deposits on the pump piston (included in Pack-PWD)
Injection
- Injection mode: liquid or solid
“Load & Go” technology: with automatic 10-way valve that allows the use of staggered dry load or loops for even more efficient injection
“RFID technology”: automatic detection of dry load and injection loops
Stainless steel injection loops 2 mL and 5 mL with RFID tag
Detection
- UV detector 200-400 nm
UV detector 200-800 nm (included in the Pack-UVextended)
“DAD” technology
Multi-wavelength and scan collection over a band of 200 nm (or 600 nm with the Pack-UVextended)
Spectral visualization and confirmation of purity
Flow cell pathway: 0.3 mm / 40 µL
Integrated ELSD detector (included in Pack-iELSD)
Column & holder compatibility
-
Normal phase (NP)
Particle sizes: 5 µm, 10 µm, 15 µm, 25 µm, 30 µm, 50 µm
Reverse phase (RP)
Particle sizes: 5 µm, 10 µm, 15 µm, 25 µm, 30 µm, 50 µmFormat : all Flash columns up to F0800 ((with the integrated column support option for the F0800 format column) & Dry-Load up to F0330 size are compatible.
all preparative column up to 80mm ID are compatible2 integrated column holders or 3 column holders (included in Pack-Multi)
2 integrated Dry-Load holdersRFID technology: automatic detection of the columns
Fraction collector
- Capacity:
– 3 racks
– 132 tubes (18 x 150 mm) - .
- Technology:
– Automatic cleaning of the fraction collector needle (to avoid cross-contamination with the previous purification)
– RFID: automatic detection of the racks installed in the fraction collector
Proposed racks
- Delivery includes a choice of 3 racks from the following list:
- ● Rack for tests tubes:
13 x 73 mm | 13 x 100 mm | 16 x 150 mm
18 x 150 mm |18 x 180 mm | 21 x 150 mm
25 x 150 mm | 28 x 150 mm | 29.5 x 200 mm - ● Rack for Schott laboratory bottles 250 mL
- ● Rack for funnels
- .
- Position of the numbered tubes (to facilitate identification of the tubes)
Software and control unit
- Control via InterSoft® X software included:
• Genius, Artificial Intelligence
• “Flash & Go”™ technology: compatible with our smartphone app “TLC to Flash & Prep”
• “Boost & Go”™ technology
Windows 10
Remote control
Touchscreen 15″
8 USB ports
2 RJ45 ports
Bluetooth/Wi-Fi key
System optimization
- Tubing after pump: 1.6 mm internal diameter
Flow cell pathway: 0.3 mm / 40 µl
Safety
- System light status
Solvent tray with drainage system
Level sensor for solvents and waste - Retention tray with leak detectors:
- – for the pump,
- – for the UV detector,
- – for the valve, Dry-Load holder,
- – for the fraction collector,
- – for the fraction iELSD (included in Pack-iELSD)
- “Fume Enclosure” technology (allows the use of the purification system without a hood)
Materials in contact with solvents
- ETFE
PTFE
316 L Stainless steel
Hastelloy
PEEK
Kalrez
Sapphire
Ruby
Kel-F
Zirconium
PPS
Nitronic 60
Borosilicate glass
Accessories box
-
Tubing with fitting & ferrules:
– Tygon tubing for solvent tray drainage
– Level sensor tubing ans waste (x5)
– Suction tubes (x4)
– SP-stainless steel adapter for injection (injection valve port 1)
– ETFE tubing 1/8″ fraction collector outlet to waste
– ETFE tubing 1/8″ draining for fraction collector
– 2 ml & 5 ml Stainless Steel loop with RFID tag5.250 Flash version:
– ETFE tubing column C2 outlet to 6-ways valve port 3
– ETFE tubing 6-ways valve port 4 to detector inlet
– ETFE tubing column C1 outlet to 6-ways valve port 5
– ETFE tubing Dryload DYL2 outlet to 10-ways valve port 2
– ETFE tubing Dryload DYL1 outlet to 10-ways valve port 10
– ETFE tubing 10-ways valve port 3 to inlet column C2
– ETFE tubing 10-ways valve port 5 to inlet Dryload DYL2
– ETFE tubing 10-ways valve port 7 to inlet Dryload DYL1
– ETFE tubing 10-ways valve port 6 to waste
– ETFE tubing 10-ways valve port 9 to inlet column C15.250 Pack-Multi Flash version:
– ETFE tubing column C1 outlet to 6-ways valve port 5
– ETFE tubing Dryload DYL2 outlet to 10-ways valve port 2
– ETFE tubing Dryload DYL1 outlet to 10-ways valve port 10
– ETFE tubing tubing 10-ways valve port 5 to inlet Dryload DYL2
– ETFE tubing 10-ways valve port 7 to inlet Dryload DYL1
– ETFE tubing 6-ways selection valve port 2 to column C3 inlet
– ETFE tubing 6-ways selection valve port 6 to column C2 inlet
– ETFE tubing 10-ways valve port 6 to waste
– ETFE tubing 10-ways valve port 9 to column C1 inlet
– ETFE tubing column C3 outlet to 6-ways selection valve port 3
– ETFE tubing column C2 outlet to 6-ways selection valve port 55.250 Prep version:
– ETFE tubing column C2 outlet to 6-ways valve port 3
– ETFE tubing column C1 outlet to 6-ways valve port 5
– PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
– PEEK tubing 10-ways valve port 3 to inlet column C2
– PEEK tubing 10-ways valve port 5 to inlet Dryload DYL2
– PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
– PEEK tubing 10-ways valve port 9 to column C1 inlet
– PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
– Adapter 5/16”-24 female to 10-32 male
– Stainless steel union 1/8″
-
5.250 Pack-Multi version Prep :
– ETFE tubing column C1 outlet to 6-ways valve port 5
– PEEK tubing Dryload DYL2 outlet to 10-ways valve port 2
– PEEK tubing tubing 10-ways valve port 5 to inlet Dryload DYL2
– PEEK tubing 10-ways valve port 7 to inlet Dryload DYL1
– PEEK tubing 6-ways selection valve port 2 to column C3 inlet
– PEEK tubing 6-ways selection valve port 6 to column C2 inlet
– PEEK tubing 10-ways valve port 9 to column C1 inlet
– PEEK tubing Dryload DYL1 outlet to 10-ways valve port 10
– PEEK tubing column C3 outlet to 6-ways selection valve port 3
– PEEK tubing column C2 outlet to 6-ways selection valve port 5
– Adapter 5/16”-24 female to 10-32 male -
– Stainless steel union 1/8″
ELSD accessories (included in Pack-iELSD)
– Reduction gear tubing 6 mm to tubing 4 mm
– Pneumatic white nylon tubing lenght 3m
– In-line filter 20 microns
– ETFE tubing from in-line filter to detector
– ETFE tubing 6-ways selection valve port 4 to in-line filterPWD accessories:
– Tubing kit PWDCables :
– RFID cable (x4)
– USB/USB cable – unit control to fraction collector
– Power cord – screen to fraction collector
– HDMI cable – screen to unit control
– USB/MINI USB cable – screen to unit control
– LED cable – unit control to fraction collector
– Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
– Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
– Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
– Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in Pack-iELSD)
– Power cord – pump to fraction collector + unit control to fraction collector (x2)
– General power cordSolvent tray
Clips P
Screw CHC M4x8
Grower M4 (x3)
Stylus with its clip
UV cell test
Rear fastening (x2)
Screws BHC 4×10 (x2)
UV trapdoor
ELSD trapdoor
Racks (x3)
Compliance report
Communication
- Communication cable Sub-D9 male/female (1 m) – unit control to fraction collector
Communication cable Sub-D9 male/female (0.5 m) – unit control to pump
Communication cable Sub-D9 female/female (1 m) – unit control to UV detector
Communication cable Sub-D9 female/female (1 m) – unit control to iELSD (included in the Pack-iELSD)
Power supply
- 110 V ou 220 V
Dimensions & Weight
- Weight : 50 kg